"I don't, I don't", she reportedly said backstage at the Globes when asked if she planned to run. She has ruled out in the past any hopes for running for president in 2020.
"I want all the girls watching here and now to know that a new day is on the horizon!"
Mr Trump's daughter, Ivanka, tweeted her support for Winfrey - though not necessarily politically.
"Acer announces their latest 11" Chromebook ahead of CES 2018
The Acer Chromebook 11 has been one of the best cheap, low-power Chromebooks you can buy for kids or students for a long time. It comes with an HDR webcam for video calling and has dual stereo speakers with an integrated microphone.
I quickly told myself nah, no way, she wouldn't.
Oprah, raised in poverty by a single mother, went on to host the top-rated talk show The Oprah Winfrey Show for 25 years before stepping down in 2011 to focus full-time on her production company Harpo Productions.
Yesterday a tweet about the Golden Globes and Oprah Winfrey was sent by a third party agency for NBC Entertainment in real time during the broadcast. Step back for a moment and consider where we've come: If I'd told you five years ago that the 2020 presidential race would be Oprah versus The Donald, you'd have insisted I seek professional help.
Wearing a black gown to show support for victims, she used her platform to promote the "Time's Up" movement against sexual harassment and assault, throwing her support behind others who have exposed sexual misconduct in Hollywood and elsewhere in politics and the media. She was cast as Sofia Johnson in the Steven Spielberg-directed film, which saw her nominated for a best supporting actress Oscar in 1986. I do think she was intrigued by the idea.
HTC Vive Introduces New Vive Pro Headset, Wireless Adapter
In related news, Valve will be showing off SteamVR Tracking 2.0 at CES this week, which will allow the use of four base stations. It also adds integrated headphones and, thankfully, still works with all the current Vive content already available.
And I have tried many, many, many times to explain what a moment like that means to a little girl, a kid watching from the cheap seats as my mom came through the door, bone exhausted from cleaning other people's houses.
"Yeah, I'll beat Oprah", Mr Trump said.
No clear Democratic front-runner has emerged to challenge Trump in 2020, with the immediate political focus in the USA on November's congressional elections, when the 435-member House of Representatives will be up for election, along with a third of the 100-member Senate.
This isn't the first time that Weight Watchers has been a beneficiary of Winfrey's clout.
Trump Suggests Hillary Clinton's Former Top Aide Huma Abedin Should Be Jailed
The report noted that Abedin forwarded State Department passwords to her personal Yahoo email account. Millions of Yahoo accounts were hacked.
Winfrey endorsed Democratic Senator Barack Obama of IL when he first ran successfully for the U.S. presidency in 2008, and former U.S. Secretary of State Hillary Clinton in her losing 2016 campaign against Trump.